Supplementary Figure 1: Additional characterisation of media converted naive hiPSCs. | Nature Methods

Supplementary Figure 1: Additional characterisation of media converted naive hiPSCs.

From: Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming

Supplementary Figure 1

(a) Experimental outline for the media conversion approach to toggle primed hiPSCs to a naive state (note: naive t2iLGöY culture condition was insufficient for conversion). (b) Resulting colony morphologies (Scale bar, 250μm) and (c) Immunofluorescence labelling for classical pluripotency markers TRA-1-60, SSEA3, NANOG, OCT4 and naive pluripotency associated markers KLF17 and TFE3 (Scale bar, 25μm).

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