Supplementary Figure 2: Additional characterisation of different naive hiPSCs. | Nature Methods

Supplementary Figure 2: Additional characterisation of different naive hiPSCs.

From: Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming

Supplementary Figure 2

(a) viSNE map (related to Fig. 1d) coloured for expression levels of cell surface markers CD24, SSEA3, SSEA4, F11R, NLGN4X, TRA-1-60. (b) Histogram of flow cytometry data for detection levels of cell surface marker F11R in primed and naive rt2iLGöY hiPSCs (representative histogram derived from n=3 cell donors 32F, 38F and 55F). (c) MDS plot of primed and different naive hiPSCs derived in this study. Two donors (32F and 55F) were used to generate all the hiPSCs except for cNHSM (one donor, 32F). Each dot represents a different cell culture sample per indicated condition. (d) Growth curves of primed and naive r5iLAF and rt2iLGöY iPSCs (n=3 culture replicates from 32F, error bars, s.e.m.). (e) Bright field image (Scale bar, 250μm) and immunofluorescence staining (Scale bar, 25μm) of primed iPSCs obtained by differentiation of naive rt2iLGöY hiPSCs, (32F).

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