Supplementary Figure 6: Conservative sorting model21 for LETM1.
From: Antibodies to biotin enable large-scale detection of biotinylation sites on proteins
(Left Panel) Many inner membrane proteins are imported via the Stop-Transfer pathway, where proteins are arrested at the TIM23 complex and laterally instead into the membrane. (Right Panel) Another, smaller class of inner membrane proteins are processed via a Conservative Sorting pathway. In this pathway, proteins initially translocate to the matrix prior to insertion in the inner membrane. We propose that biotinylation of Y141 may occur on a processing intermediate of LETM1 if the protein is processed via the Conservative Sorting pathway.
21Neupert, W. & Herrmann, J.M. Translocation of proteins into mitochondria. Annu Rev Biochem 76, 723-749 (2007).
