Supplementary Figure 7: 3D imaging of an Irganox delta layer reference material. | Nature Methods

Supplementary Figure 7: 3D imaging of an Irganox delta layer reference material.

From: The 3D OrbiSIMS—label-free metabolic imaging with subcellular lateral resolution and high mass-resolving power

Supplementary Figure 7

A) Orbitrap MS (mode 3), 5 keV Ar2000+ sputtering and analysis, intensity depth profile of the Irganox 1010 molecular ion, [C73H107O12]− at m/z 1175.776 (solid line), and fragment ion from Irganox 3114, [C33H46N3O5]− at m/z 564.344 (dashed line). B) ToF MS (mode 9), 5 keV Ar2000+ sputtering and 30 keV Bi3+ analysis intensity depth profile, as A) with 3D reconstruction from ToF MS data and C) Dual analyser, dual beam mode (10) with 5 keV Ar2000+ sputter beam. Intensity depth profiles as A) for Orbitrap MS Ar2000+ analysis beam and ToF MS 30 keV Bi3+ beam, with 3D reconstruction from ToF MS data. Results presented are from a single measurement.

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