Supplementary Figure 11: Three-dimensional multicellular consortia execute higher-order computational logics.
From: Programmable full-adder computations in communicating three-dimensional cell cultures
a) The coculture of I1 and I2 cell types forms a 3D multicellular consortium, which performs subprocesses to produce OS exclusively in the presence of IV or IDPV. Each multicellular consortium comprises a total of 10,000 cells, and fluorescence of 3D cell cultures was quantified after 42 h (Error bars represent the means ± s.d. of n=9 3D cell cultures from n=3 independent experiments); Live-cell microscopy experiments quantifying the fluorescence of 3D cell culture every 6 h are illustrated (Error bars represent the means ± s.d. of n=3 3D cell cultures of a representative experiment). Representative fluorescent micrographs illustrating the maximum intensity projections from z-stacks are shown (scale bar, 100 μm) for the coculture of I1 and I2 cell types forms a 3D multicellular consortium, which performs subprocesses to produce OS exclusively in the presence of IV or IDPV. b) The coculture of D1-D3 cell types forms the 3D multicellular consortium, which performs subprocesses to produce OC in the presence of IDP, IDV, IPV or IDPV in a WD-dependent inter-cellular communication process. Each multicellular consortium comprises a total of 10,000 cells, and fluorescence of 3D cell culture was quantified after 42 h (Error bars represent the means ± s.d. of n=9 3D cell cultures from n=3 independent experiments); Live-cell microscopy experiments quantifying the fluorescence of 3D cell culture every 6 h are illustrated (Error bars represent the means ± s.d. of n=3 3D cell cultures of a representative experiment). Representative fluorescent micrographs illustrating the maximum intensity projections from z-stacks are shown (scale bar, 100 μm) for the coculture of D1-D3 cell types forms the 3D multicellular consortium, which performs subprocesses to produce OC in the presence of IDP, IDV, IPV or IDPV in a WD-dependent inter-cellular communication process. c) Representative fluorescent micrographs illustrating the maximum intensity projections from z-stacks are shown (scale bar, 100 μm) for the coculture of H1-H4 cell types form a 3D multicellular consortium, which performs subprocesses to produce OS exclusively in the presence of ID or IP in a WH-dependent inter-cellular communication process.
