Supplementary Figure 12: Multicellular consortia H1-H4, I1-I2 and D1-D3 and full-adder 3D cell culture produce secreted reporter genes according to the corresponding truth table of higher-order logic circuits.
From: Programmable full-adder computations in communicating three-dimensional cell cultures
a) 3D cell cultures containing cell types H1-H4 secrete the reporter protein SEAP representing OS only in the presence of ID ANDNOT (IP OR IV) OR IP ANDNOT (ID OR IV). The supernatants of the same tissues as shown in Fig. 3a were used to quantify SEAP expression (n=9 tissues examined in three independent experiments). b) 3D cell cultures containing cell types I1-I2 secrete the reporter protein SEAP representing OS only in the presence of IV ANDNOT (ID OR IP) OR ID AND IP AND IV. The supernatants of the same tissues as shown in Fig. 3b were used to quantify SEAP expression (n=9 tissues performed in three independent experiments). c) 3D cell culture containing cells D1-D3 secrete the reporter protein GLuc representing OC in the presence of ID AND (IP OR IV) OR IP AND IV. The supernatants of the same tissues as shown in Fig. 3c were used to quantify GLuc expression (n=9 tissues performed in three independent experiments). d) The full-adder 3D cell culture secretes the reporter proteins SEAP representing OS and GLuc representing OC according to the truth table of the full adder. The supernatants of the same tissues as shown in Fig. 4c were used to quantify SEAP or GLuc expression (n=9 tissues examined in three independent experiments)
