Supplementary Figure 2: Design of a modular logic-gate-based computational platform
From: Programmable full-adder computations in communicating three-dimensional cell cultures
a) The AND-logic gate module produces an output only in the presence of both ITF or IR AND ISM or W. The ribozyme-optimized AND-logic gate modules are shown for all three inputs ID, IP and IV and both chemical wires WH and WD. The ANDNOT-logic gate module produces an output only in the presence of ITF ANDNOT ISM. The ribozyme-optimized ANDNOT-logic gate modules responsive to all three inputs ID, IP and IV are shown. b) A dimerization-dependent AND-logic gate module produces an output only in the presence of both IDBD-Coh2 AND IVP16-DocS, resulting in the assembly of a synthetic transcription factor. A DNA-binding domain (DBD) fused to cohesin (Coh2) strongly dimerizes with the dockerin (DocS) fused to a transactivation domain (VP16). The optimized AND-logic gate module using Gal4 as the DBD is shown (purple). Similarly, a dimerization-dependent assembly of a trigger-inducible DBD resulted in an AND ANDNOT-logic gate module that produces an output only in the presence of both IDBD-Coh2 AND IVP16-DocS ANDNOT ISM. The optimized AND ANDNOT-logic gate modules utilizing TetR, VanR and TtgR responsive to all three small-molecule inputs ID, IP and IV are shown. Error bars represent the means ± s.d. of n=3 independent experiments performed in triplicate.
