Supplementary Figure 1: BAR identifies known nuclear envelope proteins by Western blot and mass-spectrometry
From: Biotinylation by antibody recognition—a method for proximity labeling
A. Aligned signal distribution of lamin A/C (MAB3211; Cy3) and biotin (FITC-Avidin), derived from Fig 1B.
B. Western blot using Streptavidin-HRP showing antibody guided efficient biotin labeling and recovery of proteins. Beads – post-elution beads were boiled in Laemmli sample buffer to validate effective elution from the beads.
C. LAP2, a known lamin A/C binding protein, is pulled down by BAR, as evident from a Western blot.
D. A scaffold (Proteome Software, Portland, Oregon) screenshot showing multiple known NE and lamin A/C interacting proteins, and their peptide counts in the antibody sample and control.
