Supplementary Figure 2: Effect of labeling time and antibody target on BAR | Nature Methods

Supplementary Figure 2: Effect of labeling time and antibody target on BAR

From: Biotinylation by antibody recognition—a method for proximity labeling

Supplementary Figure 2

A. Protein level signal intensity (i.e. area) of 1 minute in-gel digestion vs 7 minutes on bead digestion. Proteins ordered (x-axis) in descending 7 minute intensity.

B. Heat-map showing the fraction of each dataset covered by any other dataset.

C. Heavy (lamin A/C MAB3211 antibody) to light (lamin A/C N-18 antibody) ratios of lamins, LAP2β and nuclear pore complex proteins.

D. Heavy (lamin A/C) to light (lamin B1) ratios of lamins, LAP2β and nuclear pore complex proteins.

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