Supplementary Figure 5: Ku70 and Ku80 interact with lamin A/C, as evident by FRET
From: Biotinylation by antibody recognition—a method for proximity labeling
Förster resonance energy transfer (FRET) in HeLa cells show that for Ku70 and Ku80, but not DNA-PKcs, fluorescence intensity increases after bleaching of lamin A/C adjacent fluorophores. FRET efficiency was 0 for DNA-PKcs, 0.34 for Ku70 and 0.15 for Ku80. We note that negative FRET results, as is the case for DNA-PKcs, cannot be interpreted as a lack of interaction.
