Supplementary Figure 11: Hyperspectral SRS imaging of encoded beads in live cells | Nature Methods

Supplementary Figure 11: Hyperspectral SRS imaging of encoded beads in live cells

From: Supermultiplexed optical imaging and barcoding with engineered polyynes

Supplementary Figure 11

Bright-field image shows the spatial distribution of unidentified beads in cells. Consecutive SRS imaging at characteristic frequencies of polyynes allows rapid decoding and visualization of bead identity in space. Experiments were repeated three times independently with similar results.

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