Supplementary Figure 8: An anti-HER2 EVIR promotes DC cross-dressing with MHCI/antigen complexes in a mouse tumor model.
From: EVIR: chimeric receptors that enhance dendritic cell cross-dressing with tumor antigens
(a-c) MC38 cancer cells (either H2kb wild-type or KO) and H2kb-deficient DCs (FVB/n origin) were co-mingled in matrigel plugs implanted in H2kb-deficient NSG mice (see Supplementary Figure 7c) and the tumors allowed to grow for 8 days. A schematic representation of the experiment is shown in (a). Panel (b) shows representative flow cytometry analyses of H-2Kb expression in the relevant cell types: dLNGFR+ cells, which represent transduced DC-EVIR or DC-CtrlR cells, and dLNGFR-negative CD45+ cells, which represent non-transduced hematopoietic cells recruited to the matrigel plug from the NSG host. Panel (c) shows analysis of the flow cytometry data. Data show mean values ± SEM (n=4 mice/condition) after normalizing the data to H-2Kb expression in the dLNGFR-negative cells (set to 1); statistical analysis by two-way ANOVA with Sidak's multiple comparison test.
(d) Representative flow cytometry dot plots showing the gating strategy employed in (a-c).
Numerical values for the experiments with quantitative data are presented in Supplementary Table 2.
