Supplementary Figure 11: An anti-HER2 EVIR promotes MHCI recycling in DCs.
From: EVIR: chimeric receptors that enhance dendritic cell cross-dressing with tumor antigens
Representative confocal images of H-2Kb-negative DC-EVIR incubated with PKH67-labeled EV-HER2 (green), which were isolated from either H2kb-proficient (cells 1-4 and 6-9) or KO (cell 5) MC38 cells. Additional examples (including H2kb KO EVs assayed at 24 h) are shown in Figure 2c. The cells were analyzed 5 h (left panels) or 24 h (right panels) after exposure to the EVs. The cells were immunostained with antibodies against H-2Kb (magenta) and dLNGFR (white); nuclei are stained with DAPI (blue). Scale bar, 10 μm. Images are representative of 10 randomly selected cells per condition. Results are representative of 2 independent experiments.
