Supplementary Figure 5: DC-EVIR cells promote T cell proliferation.
From: EVIR: chimeric receptors that enhance dendritic cell cross-dressing with tumor antigens
(a) Flow cytometry analysis of CD8+ OT-I proliferation, assessed by CellTrace dilution, analyzed after their co-culture with DC-CtrlR or DC-EVIR cells exposed to EV-HER2/OVA (top panels) or EV-OVA (bottom panels). Data show representative flow cytometry histograms of 3 independent cell cultures per condition (see Figure 1e for quantitative data).
(b) Flow cytometry analysis of CD8+ OT-I proliferation, assessed by CellTrace dilution, analyzed after treatment with EV-HER2/OVA in the absence of DCs, or after co-culture with DC-EVIR or DC-CtrlR cells and without EVs. Data show representative flow cytometry histograms of 3 independent cell cultures per condition.
(c) Dose-response of CD8+ OT-I cell proliferation, assessed by CellTrace dilution, after co-culture with DC-EVIR or DC-CtrlR in the presence of EV-HER2/OVA (left) or EV-OVA (right). Data show mean values ± SEM (n=3 independent cell cultures per condition). Statistical analysis by two-way ANOVA with Sidaks's multiple comparison test.
Numerical values for the experiments with quantitative data are presented in Supplementary Table 2.
