Supplementary Figure 5: Pan-neuronal mime-seq is highly reproducible and robust to Ath-HEN1 dose.
From: Cell-type specific sequencing of microRNAs from complex animal tissues
(a) Western blot analysis of the expression of MYC-Ath-HEN1 driven under three different pan-neuronal promoters in C. elegans (biological duplicates are shown). Tubulin detection was used for loading control. Non-transgenic, N2 animals were used for negative control. For full scans of the blots see Suppl. Fig. 10. Independent repeats = 2. (b, d, f) Comparison of the relative abundances of all confidently detected (>10 cpm in the average of all untreated samples) mature miR strands in henn-1(0) worm L1s expressing Ath-HEN1 under three different pan-neuronal drivers, before and after oxidation treatment, shows that some miRNAs are enriched while others are depleted by the oxidation treatment. (c, e, g) Comparison of the relative abundances of all confidently detected (>10 cpm in the average of all untreated samples) miRNAs for each pair of biological replicates, for both treated and untreated samples, in all three pan-neuronal experiments. Spearman correlation coefficients and their significance are shown. All experiments in this figure were performed on two biologically independent samples.
