Supplementary Figure 6: Methylated miR* enrichment supports neuronal miRNA assignments.
From: Cell-type specific sequencing of microRNAs from complex animal tissues
For each pan-neuronal mime-seq experiment, we analyzed the change in relative abundance after periodate-mediated oxidation of all miR strands detected above an average of 10 cpm in all the untreated samples, as well as of all miR* strands corresponding to the detected miRs, regardless of their abundance. The correlations between the enrichment of both species is plotted for each experiment and the Pearson correlation coefficients (rp) and associated two-tailed p-Values (calculated in Prism7) are shown. The Venn diagrams display how many miR strands were significantly enriched (log2 fold-change > 1; p-Value<10−3) upon oxidation in the respective experiment and from their respective miR* strands, how many were also enriched (log2 fold-change > 1; p-Value<0.0013) and expressed above 10 cpm. The majority of miR* strands that do not support miR enrichment are expressed below 10 cpm in the unoxidized samples. Experiments in this figure were performed on two biologically independent samples.
