Supplementary Figure 7: Mime-seq reveals the miRNomes of C. elegans tissues of diverse composition and size.

(a) Western blot analysis of the expression of MYC-Ath-HEN1 driven under different tissue-specific promoters in C. elegans (biological duplicates are shown). Tubulin detection was used for loading control. Non-transgenic, N2 animals were used for negative control. For full scans of the blots see Suppl. Fig. 10. Independent repeats = 2 for elt-2 and myo-2, 1 for myo-3. (b, d, f) Comparison of the relative abundances of all confidently detected (>10 cpm in the average of all untreated samples) mature miR strands in henn-1(0) worm L1s expressing Ath-HEN1 under three different tissue-specific drivers, before and after oxidation treatment, shows that some miRNAs are enriched while others are depleted by the oxidation treatment. (c, e, g) Comparison of the relative abundances of all confidently detected (>10 cpm in the average of all untreated samples) mature miR strands for each pair of biological replicates, for both treated and untreated samples, in all three tissue-specific experiments. Spearman correlation coefficients and their significance are shown. All experiments in this figure were performed on two biologically independent samples.