Supplementary Figure 4: Ath-HEN1-directed methylation followed by oxidation-dependent cloning preserves the relative quantity of miR and miR* species.
From: Cell-type specific sequencing of microRNAs from complex animal tissues
Relative abundance of the indicated number of fly miR (red) and miR* (blue) species in the indicated small RNA libraries generated as described in Fig. 2a. Only miR and miR* strands that were significantly enriched (log2 fold-change > 1; p-Value<10−3) upon oxidation in the respective input dilution, as determined in Fig. 2b and Suppl. Fig. 3a and c, are shown. Spearman correlation coefficient (rs) and associated p-Value were determined separately for miR (red) and miR* (blue) species. Mean±stdev of three independent experiments are shown.
