Supplementary Figure 10: Predicted MHC I binding affinities.
From: Improved Ribo-seq enables identification of cryptic translation events
(a) We used NetMHCcons [Karosiene et al., 2012] to predict the binding affinities of all identified peptides to the respective HLA allotypes for both MHC-1 peptidome data sets (published: HLA-A*03:01, HLA-A*23:01, HLA-B*08:01, HLA-B*15:01, HLA-C*07:02, HLA-C*07:04; new: HLA-A*01:01, HLA-A*03:01, HLA-B*08:01, HLA-B*51:01, HLA-C*07:01, HLA-C*01:02). Shown is the percentile rank of the IC50 value, as predicted by NetMHCcons. Strong binders are recognized by a percentile rank ≤ 1 (vertical dotted line) [Karosiene et al., 2012]. With the exception of Rp-Bp predicted peptides in the new data set, all predictions of novel ORFs result in peptides that strongly resemble the binding affinities of peptides from annotated proteins.
