Supplementary Figure 4: Signal-to-noise ratio of read classes.
From: Improved Ribo-seq enables identification of cryptic translation events
Read classes (characterized by read length and 5’ mismatch state) exhibit specific signal to noise ratios from slightly above the theoretical minimum (dashed horizontal line) to more than 8. Ignoring 5’ mismatches (pale orange) increases noise levels. For this experiment, the optimal deterministic mapping strategy utilizes four read classes, namely 28 and 29 bp without 5’ mismatch, and 29 and 30 bp long reads with 5’ mismatch.
