Supplementary Figure 5: Improved signal-to-noise with PRICE.
From: Improved Ribo-seq enables identification of cryptic translation events
(a) Deterministic mapping approaches trade off signal against signal to noise ratio (connected dots, from left to right) when considering reads of additional sizes. Probabilistic mapping and regularization (symbols for the regularization parameter δ) substantially improve Ribo-seq data quality for a broad range of published data sets (see Supplementary Table 1). (b) Aggregated reads around start codons demonstrate the improved signal to noise ratio of PRICE for both the HSV-1 and HCMV data set. Codon counts were normalized to the start codon for all well-translated ORFs (at least one read per codon on average) and aggregated per position relative to the start codon.
