Supplementary Figure 1: HuProt proteins are in native conformation.
From: A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors
HuProt proteins are in native conformation. (a) Categories of proteins based on GO annotation as represented on HuProt compared to the entire human proteome as per Uniprot. (b) Top panels. Anti-ACO2 6D1BE4 mAb (Catalog #ab110320, Abcam), which recognizes a conformational specific epitope, tested for target binding on a native HuProt (left) and HuProt denatured with 9M Urea and 5 mM DTT (right). Bottom panels. Anti-SMAD4 mAb (CDI Labs, #R516.2.1G11), which recognizes a linear epitope, tested for target binding on native HuProt (left) and denatured HuProt (right). Data shown here is representative of three independent technical replicates. (c,d) A conformation-specific anti-GST antibody (CDI Labs, #27.3.6G8) tested for binding to GST-tagged proteins on the native HuProt (c, top) and denatured HuProt (c, bottom).(d) Scatter plot of signal intensity measured with the conformation-specific anti-GST antibody (CDI Labs, #27.3.6G8) in native versus denatured HuProt (e) Micrographs representing the HuProt signal observed for known Xist interaction partner (HNRNPC) and potential new partners identified in our screen (RBM46 and ELAVL2). (f) Scatter plot of signal intensity measured with Xist probe on native HuProt versus a denatured HuProt.
