Supplementary Figure 2: Competitive IP analysis.
From: A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors
Commercially sourced antibodies when tested on HuProt exhibit interactions with targets other than the intended target. These antibodies also interact with the off-targets in a competitive immunoprecipitation experiment. Rank, z and S-score for off-targets represented here are indicated in Table S5. Antibodies tested are as follows: (a,b) Novus Biological anti-RELA (catalog# NB100-56055, lot# AB071609E) and PCRP anti-RELA (#YP268.1.2B6). (c) Santa Cruz Biotechnology anti-FOSL1 (Catalog# SC-28310, Lot# I1415) and PCRP anti-FOSL1 (clone ID# R1024.1.1G1). (d,e) Cell Signaling Technologies anti-FOSL1 (Catalog#5281, Lot#2) and PCRP anti-FOSL1 (#R1024.1.1G1). (f) Abgent anti-USF2 (Catalog# AT4478a, Lot# 11189) and PCRP anti-USF2 (clone ID# R1156.1.1A7). (g) LifeSpan Biotechnology anti-ZEB2 (Catalog# LS-C175748, Lot# 51937). * indicated in the immunoblots refer to unidentified off-targets detected by the commercial mAbs.
