Supplementary Figure 9: Test of the robustness of the feedback system at different temperatures. | Nature Methods

Supplementary Figure 9: Test of the robustness of the feedback system at different temperatures.

From: Burden-driven feedback control of gene expression

Supplementary Figure 9

GFP (top) and VioB-mCherry (bottom) production rate per cell are shown when different levels of arabinose are used to trigger VioB-mCherry production in DH10B cells at 37°C and 30°C. The performance of the feedback system in presence of construct pSB1C3-H2 (Closed-Loop) was compared to an ‘Open-Loop’ version where the sgRNA targeting pBAD had been replaced by a random sgRNA with no affinity for any promoter. For both strains VioB-mCherry output from pSB1C3-H2 (lower panels) and cellular capacity (top panels) were characterised when different levels of arabinose were used to induce construct expression. Dots show single repeats while bars show the mean value. Error bars show standard deviation of three independent repeats.

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