Supplementary Figure 11: The biomolecular feedback controller retargeted to a different synthetic construct.

Growth rate (left), GFP expression rate (middle) and total luminescence per cell (right) are shown for a construct where the firefly luciferase operon is expressed under the control of the lacP promoter (lacP-Luc) in DH10B E. coli. In cells where the lacP-targeting closed-loop feedback controller is alongside the luciferase operon plasmid (FB), luminescence is decreased while growth rate and GFP production rate are improved. Sequences of the lacP core promoter and sgRNA targeting this are both shown in Table S7. Dots show single repeats while bars show the mean value. Error bars show standard deviation of three independent repeats.