Supplementary Figure 13: Presence of the feedback controller confers robustness to varying concentrations of inducer.
Top and bottom panels show VioB-mCherry and GFP production rates 1 hour post induction at different arabinose concentrations, respectively, in DH10B cells. While pSB1C3-H2 alone (H2) is highly sensitive to increasing concentrations of arabinose, pSB1C3-H2 with the feedback controller plasmid (FB) is capable of maintaining the GFP production rate per cell (i.e. the host capacity) and the VioB-mCherry production rates per cell within a much narrower range, thereby demonstrating robustness of the feedback system to inducer fluctuations. Dots represent the mean and the error bars indicate the maximum and minimum values of the triplicate.
