Supplementary Figure 15: Single-cell 24-h time-course experiment of the burden-driven feedback controls.

Graphs show single-cell flow cytometry measurements of the population corresponding to the plate reader data shown in Figure 3d. The geometric mean values of sfGFP and VioB-mCherry expression per cell are given next to the histogram distributions. Plots confirm that no escape mutant arose during the batch culture dynamic experiment. The experiment was repeated three times with similar results.