Supplementary Figure 2: Correlations between RNA-seq data and the in vivo burden assay.
Left panel shows the relationship between the total RNA harvested during RNAseq sample prep and the total cells grown for each sample in the plate-based burden assay done in parallel. Correlation is given as R2 - the square of the Pearson product moment correlation coefficient. Cell concentration per well was determined by OD600 measurement at each time point (15 min and 60 min post-induction). RNA concentrations from extractions into the same volume were determined by spectrographic measurement with Nanodrop. Data represent values from three independent repeats. Right panel shows the relationship between RNA-seq GFP FPKM (x-axis) and in vivo GFP production rate per cell (y-axis). Correlation is given as R2 - the square of the Pearson product moment correlation coefficient. Data are from 15 and 60 min post-induction. Control samples are the strains (DH = DH10B, MG = MG1655) with the three empty plasmids (pSB1C3, pLys and pD864). Burden samples are the strains with the four synthetic constructs (pSB1C3-Lux, pSB1C3-H3, pLys-M1 and pD864-LacZ). Data show the mean values for each condition.
