RNA amplification and cDNA synthesis for qRT-PCR directly from a single cell

Abstract

Gene expression studies by quantitative reverse-transcription PCR (qRT-PCR) can be challenging when attempted with samples of a few cells or a single cell. Epicentre's MessageBOOSTER^™ cDNA Synthesis from Cell Lysates kit enables RNA amplification and cDNA synthesis directly from cell lysates. A MessageBOOSTER kit reaction from a single cell produces enough cDNA for thousands of qPCRs, even with low-abundance messages.