Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Advertising Feature: Application Note
  • Published:

Real-time, label-free monitoring of cellular invasion and migration with the xCELLigence system

Nature Methods volume 6, pages v–vi (2009)Cite this article

Abstract

For researchers performing invasion and migration assays, the CIM-Plate 16 used with the xCELLigence RTCA DP Instrument from Roche Applied Science offers a means to monitor cellular responses in real time, without exogenous labels, through impedance-based technology. The CIM-Plate 16 provides a kinetic cell-response profile throughout an experiment, detailing the onset and rate of invasion and migration. This information can help researchers understand processes that current endpoint experiments are unable to address.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to the full article PDF.

USD 39.95

Prices may be subject to local taxes which are calculated during checkout

Figure 1: The CIM-Plate 16 device for real-time cell invasion/migration analysis.
Figure 2: Migration kinetics of HeLa cells, as shown by continuous monitoring of live-cell migration.
Figure 3: Invasion and migration kinetics of HT1080 cells in indicated dilutions of Matrigel using FBS as the chemoattractant.

References

  1. Solly, K., Wang, X., Xu, X., Strulovici, B. & Zheng, W. Application of real-time cell electronic sensing (RT-CES) technology to cell-based assays. Assay Drug Dev. Technol. 2, 363–372 (2004).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Roche Diagnostics, Roche Applied Science, Indianapolis, Indiana, USA

    Christopher Bird & Shelli Kirstein

Authors
  1. Christopher Bird
    View author publications

    Search author on:PubMed Google Scholar

  2. Shelli Kirstein
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Shelli Kirstein.

Additional information

Disclaimer

This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the information provided.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bird, C., Kirstein, S. Real-time, label-free monitoring of cellular invasion and migration with the xCELLigence system. Nat Methods 6, v–vi (2009). https://doi.org/10.1038/nmeth.f.263

Download citation

  • Issue date:

  • DOI: https://doi.org/10.1038/nmeth.f.263

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing