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Targeted resequencing for large-scale genomic studies: accelerating NGS throughput using molecular barcodes

Nature Methods volume 7, pages i–ii (2010)Cite this article

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Abstract

Next-generation sequencing (NGS) is a powerful tool for the massively parallel investigation of specific genomic loci. However, sequence capture methodologies are required for time- and cost-effective sequence analysis in high-throughput genomic projects. HybSelect is a microarray-based sequence capture strategy that enables selective enrichment of a desired target region with increased sample numbers. Adding sample barcoding technology to the automated HybSelect approach further increases sample throughput and efficiently amends NGS technologies involved in large-scale discovery studies.

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Figure 1: High levels of multiplexing achieved by combining HybSelect™ parallel sample processing capabilities with bar-coding reagents.
Figure 2
Figure 3: Sixteen-plex parallel analysis of breast cancer genes reveals even distribution of coverage over all 16 barcoded samples.

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  1. febit biomed GmbH, Heidelberg, Germany

    Julia Lange

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  1. Julia Lange
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Correspondence to Julia Lange.

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This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the information provided.

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Lange, J. Targeted resequencing for large-scale genomic studies: accelerating NGS throughput using molecular barcodes. Nat Methods 7, i–ii (2010). https://doi.org/10.1038/nmeth.f.305

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