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mosquito® LCP: Making membrane protein crystallization accessible to the research scientist

Nature Methods volume 8, pages i–ii (2011)Cite this article

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Abstract

mosquito® LCP offers high throughput, high accuracy and unrivaled reproducibility to an otherwise challenging manual process. Its inherent flexibility, which allows it to perform the other vapor-diffusion methods for crystallography, provides the scientist with a wide range of options to suit any specific project. Its precision, reliability and ease of use offer accessibility of this novel membrane-protein crystallization technique to a wider community.

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Figure 1: The variation of dispensing accuracy from the mosquito LCP syringe is <10%.
Figure 2: Precise and repeatable positioning of drops in each well using TTP LabTech's mosquito® LCP.
Figure 3: Crystallization of β-adrenergic receptor crystals using TTP LabTech's mosquito® LCP.

References

  1. Landau, E.M. & Rosenbusch, J.P. Lipidic cubic phases: a novel concept for the crystallization of membrane proteins. Proc. Natl. Acad. Sci. USA 93, 14532–14535 (1996).

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Author information

Authors and Affiliations

  1. TTP LabTech, Melbourn, Cambridge, UK

    Wendy Gaisford

  2. Paul Scherrer Institute, Villigen, Switzerland

    Gebhard Schertler

  3. Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK

    Pat Edwards

Authors
  1. Wendy Gaisford
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  2. Gebhard Schertler
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  3. Pat Edwards
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Corresponding author

Correspondence to Wendy Gaisford.

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This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the information provided.

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Gaisford, W., Schertler, G. & Edwards, P. mosquito® LCP: Making membrane protein crystallization accessible to the research scientist. Nat Methods 8, i–ii (2011). https://doi.org/10.1038/nmeth.f.345

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  • DOI: https://doi.org/10.1038/nmeth.f.345

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