Abstract
The Illumina® Expression BeadChip® arrays are the preferred system for array-based gene expression studies because of their low input requirement, high sensitivity, low cost and high throughput. Two target labeling procedures—the TargetAmp™-Nano and TargetAmp™-Pico Target Labeling Kits for Illumina Expression BeadChip arrays—generate microgram amounts of biotin-antisense RNA (aRNA) from nanogram and picogram amounts of RNA, respectively, and yield high-quality expression analysis results.
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References
Van Gelder, R.N. et al. Amplified RNA synthesis from limited quantities of heterogeneous cDNA. Proc. Natl. Acad. Sci. USA 87, 1663–1667 (1990).
Acknowledgements
We thank A. Khanna and A. Radek (Epicentre) and I. Lewis and B. Applegate (Illumina, Inc., San Diego, California, USA) for providing data used in this application note.
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This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the information provided.
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Pease, J. Robust target labeling from small amounts of RNA for Illumina® Expression BeadChip® arrays. Nat Methods 8, i–ii (2011). https://doi.org/10.1038/nmeth.f.346
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DOI: https://doi.org/10.1038/nmeth.f.346
