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Expresso® CMV system: effortless mammalian expression cloning

Nature Methods volume 9, pages iii–iv (2012)Cite this article

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Abstract

The Expresso® systems dramatically increase the speed and efficiency of target gene cloning and protein expression. With Expressioneering™, PCR products are cloned instantly and directionally into pre-processed mammalian expression vectors without sample cleanup or enzyme treatment.

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Figure 1: Expressioneering technology for efficient, high-speed cloning and protein expression.
Figure 2: Recombinant protein expression using the Expresso CMV system: comparison of pME-HA to pcDNA3.1.
Figure 3: Expression of GPCRs using the Expresso CMV system.

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  1. Lucigen Corporation, Middleton, Wisconsin, USA

    Saurabh Sen, Heather Sternard, Eric Steinmetz & Ronald Godiska

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  1. Saurabh Sen
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  2. Heather Sternard
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  3. Eric Steinmetz
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  4. Ronald Godiska
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Correspondence to Saurabh Sen.

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Sen, S., Sternard, H., Steinmetz, E. et al. Expresso® CMV system: effortless mammalian expression cloning. Nat Methods 9, iii–iv (2012). https://doi.org/10.1038/nmeth.f.356

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