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Peggy: size- or charge-based western blotting at the push of a button

Abstract

Western blotting continues to be the most popular method used for protein analysis, in spite of its drawbacks in quantitation and reproducibility and its laborious workflow. Protein Simple's platform of Simple Western products provides a new standard in instrumentation that fully automates the archaic western blotting process and provides highly reproducible and quantitative data. Now Peggy, the newest addition to the Simple Western family, provides immunoassays with separation by size or charge in a single instrument.

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Figure 1: Size and charge data on normal PBMCs.
Figure 2: Comparison of autoimmune and normal PBMCs with size and charge assays.

References

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Correspondence to Erik Gentalen.

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Gentalen, E., White, T. & Proctor, J. Peggy: size- or charge-based western blotting at the push of a button. Nat Methods 10, i–ii (2013). https://doi.org/10.1038/nmeth.f.365

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