Abstract
The Expresso® Solubility and Expression Screening System uses a simple ligase-free cloning strategy and a single PCR product to construct and test seven distinct protein fusion partners in parallel over the course of a few days. This multiplexed system simplifies the screening of fusion partners and greatly increases the chances of obtaining improved expression and solubility of target proteins.
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Acknowledgements
This work was supported by a Small Business Innovation Research grant from the US National Institute of General Medical Sciences.
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Maffitt, M., Auldridge, M., Sen, S. et al. Rapid screening for protein solubility and expression. Nat Methods 12, i–ii (2015). https://doi.org/10.1038/nmeth.f.382
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DOI: https://doi.org/10.1038/nmeth.f.382
