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Single RNA polymerases on the stage

Nature Methods volume 3, page 3 (2006)Cite this article

Abstract

Two different single-molecule assays allowed researchers to answer long-standing questions about Escherichia coli RNA polymerase (RNAP) elongation — its mechanism and σ70 content.

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Figure 1: The 'dumbbell' assay.

References

  1. Abbondanzieri, E.A. et al. Direct observation of base-pair stepping by RNA polymerase. Nature 438, 460–465 (2005).

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  2. Kapanidis, A.N. et al. Retention of transcription initiation factor σ70 in transcription elongation: single-molecule analysis. Mol. Cell 20, 347–356 (2005).

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Authors
  1. Irene Kaganman
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Kaganman, I. Single RNA polymerases on the stage. Nat Methods 3, 3 (2006). https://doi.org/10.1038/nmeth0106-3

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  • DOI: https://doi.org/10.1038/nmeth0106-3

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