Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Advertising Feature: Application Note
  • Published:

The Overnight Express Autoinduction System: High-density cell growth and protein expression while you sleep

Nature Methods volume 2, pages 233–235 (2005)Cite this article

Abstract

The Overnight Express Autoinduction Systems enable regulated protein expression in Escherichia coli without the need to monitor the culture or add inducer during cell growth and are marketed exclusively through the Novagen brand of EMD Biosciences, Inc. (EMD Biosciences). EMD Biosciences is a major supplier of premium tools for proteomics and disease pathway analysis known through its global brands Calbiochem, Novabiochem and Novagen and is part of the Life Science and Analytics (LSA) division of Merck KGaA (Darmstadt, Germany). The company operates as EMD Biosciences, Inc. in North America and as Merck Biosciences outside North America.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to the full article PDF.

USD 39.95

Prices may be subject to local taxes which are calculated during checkout

Figure 1: SDS-PAGE analysis of crude and purified proteins from cultures autoinduced with Overnight Express System 1 or induced by IPTG.
Figure 2: Comparison of protein expression from Overnight Express Instant TB Medium prepared by autoclave or microwave.
Figure 3: Mass spectrometry analysis showing Se-Met incorporation in cells grown with Overnight Express System 2.

Notes

  1. aThe products and technologies presented in this article were developed and are offered under the terms of a license agreement between EMD Biosciences, Inc., Novagen Brand and Brookhaven Science Associates, covering US patent properties entitled “High Density Growth of T7 Expression Strains with Auto-Induction Option,” filed March 14, 2003, in the name of F. William Studier and assigned to Brookhaven Science Associates, LLC, Upton, New York 11973, USA. The Autoinduction Media Technology embodied in the Overnight Express Autoinduction Systems is based on technology developed at Brookhaven National Laboratory under contract with the US Department of Energy and is the subject of patent applications assigned to Brookhaven Science Associates, LLC. This product is to be used for research purposes only. A separate license is required for any commercial manufacture or use, including the manufacture of protein products for use in the screening of compound libraries. Information about commercial licenses may be obtained from the Office of Intellectual Property and Industrial Partnerships, Brookhaven National Laboratory, Bldg. 475D, P.O. Box 5000, Upton, New York 11973-5000, USA; telephone (631) 344-7134.

References

  1. Grabski, A., Mehler, M. & Drott, D. Unattended high-density cell growth and induction of protein expression with the Overnight Express Autoinduction System. inNovations 17, 3–6 (2003).

    Google Scholar 

  2. Studier, F.W. Protein production by autoinduction in high-density shaking cultures. Protein Expr. Purif. (in the press; 2005).

  3. EMD Biosciences, Inc. pET System Manual 10th edn., 21 (2003).

  4. Grossman, T.H., Kawasaki, E.S., Punreddy, S.R. & Osburne, M.S. Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability. Gene 209, 95–103 (1998).

    Article  CAS  Google Scholar 

  5. Overnight Express Autoinduction System 2. inNovations 18, 26 (2003).

Download references

Author information

Authors and Affiliations

  1. EMD Biosciences, Inc., 441 Charmany Dr., Madison, 53719, Wisconsin, USA

    Anthony Grabski, Mark Mehler & Don Drott

Authors
  1. Anthony Grabski
    View author publications

    Search author on:PubMed Google Scholar

  2. Mark Mehler
    View author publications

    Search author on:PubMed Google Scholar

  3. Don Drott
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Anthony Grabski.

Additional information

Disclaimer

This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the information provided.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Grabski, A., Mehler, M. & Drott, D. The Overnight Express Autoinduction System: High-density cell growth and protein expression while you sleep. Nat Methods 2, 233–235 (2005). https://doi.org/10.1038/nmeth0305-233

Download citation

  • Issue date:

  • DOI: https://doi.org/10.1038/nmeth0305-233

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing