An optimized transcription activator–like effector (TALE) and an improved assembly method promise efficient genome editing and transcriptome modulation.
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References
Miller, J.C. et al. A TALE nuclease architecture for efficient genome editing. Nat. Biotechnol. 29, 143–148 (2011).
Zhang, F. et al. Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription. Nat. Biotechnol. 29, 149–153 (2011).
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Rusk, N. TALEs for the masses. Nat Methods 8, 197 (2011). https://doi.org/10.1038/nmeth0311-197
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DOI: https://doi.org/10.1038/nmeth0311-197