Researchers describe a method called bleach-chase to quantitatively measure the half-lives of fluorescently tagged proteins in human cancer cells.
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References
Eden, E. et al. Proteome half-life dynamics in living human cells. Science advance online publication (13 January 2011).
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Doerr, A. A day in the half-life of a protein. Nat Methods 8, 201 (2011). https://doi.org/10.1038/nmeth0311-201
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DOI: https://doi.org/10.1038/nmeth0311-201