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qPCR: quicker and easier but don't be sloppy

Nature Methods volume 8, pages 207–212 (2011)Cite this article

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Gene profiling using quantitative PCR is becoming higher throughput, but researchers must be careful in gathering their data.

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References

  1. Uhlmann, V. et al. Mol. Pathol. 55, 84–90 (2002).

    Article  CAS  Google Scholar 

  2. Taylor, S., Wakem, M., Dijkman, G., Alsarraj, M. & Nguyen, M. Methods 50, S1–S5 (2010).

    Article  CAS  Google Scholar 

  3. Bustin, S.A., et al. Clin. Chem. 55, 611–622 (2009).

    Article  CAS  Google Scholar 

  4. Bustin, S.A., et al. BMC Mol. Biol. 11, 74 (2010).

    Article  Google Scholar 

  5. Vermeulen, J. et al. Nuc. Acids Res. (in the press).

  6. Vandesompele, J. et al. Genome Biol. 3, 0034–0034.11 (2002).

    Article  Google Scholar 

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  1. Monya Baker is technology editor for Nature and Nature Methods,

    Monya Baker

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  1. Monya Baker
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Correspondence to Monya Baker.

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Baker, M. qPCR: quicker and easier but don't be sloppy. Nat Methods 8, 207–212 (2011). https://doi.org/10.1038/nmeth0311-207

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