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Frankenstein's protein

Nature Methods volume 2, page 408 (2005)Cite this article

Abstract

Three polypeptide segments can be spliced together in a one-pot reaction with the aid of two orthogonal split inteins.

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Figure 1: Tandem trans-splicing of three polypeptide segments via two orthogonal split inteins.

References

  1. Shi, J. & Muir, T.W. Development of a tandem protein trans-splicing system based on native and engineered split inteins. J. Am. Chem. Soc. 127, 6198–6206 (2005).

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  2. Mootz, H.D. & Muir, T.W. Protein splicing triggered by a small molecule. J. Am. Chem. Soc. 124, 9044–9045 (2002).

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Authors
  1. Allison Doerr
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Doerr, A. Frankenstein's protein. Nat Methods 2, 408 (2005). https://doi.org/10.1038/nmeth0605-408

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  • DOI: https://doi.org/10.1038/nmeth0605-408

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