Abstract
Overexpression of an enzyme that degrades Eschericia coli mRNA leaves lots of room for the protected expression of exogenous proteins, and makes worries about background a thing of the past.
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References
Suzuki M et al. Single protein production in living cells facilitated by an mRNA interferase. Mol. Cell 18, 253–261 (2005).
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Rusk, N. Putting suicide genes to work. Nat Methods 2, 410 (2005). https://doi.org/10.1038/nmeth0605-410a
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DOI: https://doi.org/10.1038/nmeth0605-410a