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An affinity for new proteins

Nature Methods volume 3, page 584 (2006)Cite this article

Abstract

Metabolic incorporation of an unnatural amino acid allows researchers to rapidly affinity purify newly synthesized proteins from mammalian cell lysates for direct identification by mass spectrometry.

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Figure 1: Overview of the BONCAT strategy.

References

  1. Dieterich, D.C . et al. Selective identification of newly synthesized proteins in mammalian cells using bioorthogonal noncanonical amino acid tagging (BONCAT). Proc. Natl. Acad. Sci. USA 103, 9482–9487 (2006).

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Authors
  1. Allison Doerr
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Doerr, A. An affinity for new proteins. Nat Methods 3, 584 (2006). https://doi.org/10.1038/nmeth0806-584a

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  • DOI: https://doi.org/10.1038/nmeth0806-584a

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