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STEDy progress

Abstract

Continuing progress in the development of stimulated emission depletion microscopy (STED) is slowly but surely fulfilling the method's promise of ultrahigh-resolution fluorescence microscopy and may bring unexpected benefits to other imaging modalities as well.

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Figure 1: Side-by-side comparison of confocal and STED microscopy images.

References

  1. Donnert, G. et al. Macromolecular-scale resolution in biological fluorescence microscopy. Proc. Natl. Acad. Sci. USA 103, 11440–11445 (2006).

    Article  CAS  Google Scholar 

  2. Kittel, R.J. et al. Bruchpilot promotes active zone assembly, Ca2+ channel clustering, and vesicle release. Science 312, 1051–1054 (2006).

    Article  CAS  Google Scholar 

  3. Willig, K.I. et al. STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis. Nature 440, 935–939 (2006a).

    Article  CAS  Google Scholar 

  4. Willig, K.I. et al. Nanoscale resolution in GFP-based microscopy. Nat. Methods 3, 721–723 (2006b).

    Article  CAS  Google Scholar 

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Evanko, D. STEDy progress. Nat Methods 3, 661 (2006). https://doi.org/10.1038/nmeth0906-661

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  • DOI: https://doi.org/10.1038/nmeth0906-661

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