Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Advertising Feature: Application Note
  • Published:

Solving the multiplexing–dynamic range conundrum with Diffractive Optics Technology (dot™)

Abstract

The ability of the dotLab™ System to concurrently perform monitoring of protein-protein interactions in real-time, while using different assay formats, overcomes the traditional limitations of multiplexed end-point assays.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Figure 1: dotLab basics.
Figure 2: Schematic representation of each complex formed.
Figure 3: Concurrent detection of analytes over six orders of dynamic range.

References

  1. Borisenko, V. et al. Diffractive optics technology: a novel detection technology for immunoassays. Clin. Chem. 52, 2168–2170 (2006).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We recognize D. Wong of Axela Biosensors, Inc. for his assistance in developing this application note.

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Jean-Francois Houle or Sriram Kumaraswamy.

Additional information

Disclaimer

This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the information provided.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Houle, JF., Kumaraswamy, S. Solving the multiplexing–dynamic range conundrum with Diffractive Optics Technology (dot™). Nat Methods 4, i–ii (2007). https://doi.org/10.1038/nmeth1000

Download citation

  • Issue date:

  • DOI: https://doi.org/10.1038/nmeth1000

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing