Abstract
ProPur™ affinity spin columns contain a unique flow regulator, FlowGo, which maximizes the contact time between the target protein and the resin. ProPur columns offer the speed of a spin column with the yields and purities of a gravity column or a batch protocol. They are ideal for rapid small-scale and pilot purifications of His-tagged proteins, method development, expression trials, solubility determination and separation of the proteolytically cleaved His tag from the purified protein.
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References
Porath, J., Carlson, J., Olsson, I. & Belfrage, G. Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258, 598–599 (1975).
Porath, J. & Olin, B. Immobilized metal ion affinity adsorption and immobilized metal ion affinity chromatography of biomaterials. Serum protein affinities for gel-immobilized iron and nickel ions. Biochemistry 22, 1621–1630 (1983).
Porath, J. IMAC-immobilized metal ion affinity–based chromatography. Trends Anal. Chem. 7, 254–259 (1988).
Sulkowski, E. The saga of IMAC and MIT. Bioessays 10, 170 (1989).
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Brownleader, M. ProPur™: His-tagged protein purification made easy. Nat Methods 4, i–ii (2007). https://doi.org/10.1038/nmeth1004
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DOI: https://doi.org/10.1038/nmeth1004
