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New approach to complete automation in sizing and quantitation of DNA and proteins by the Automated Lab-on-a-Chip Platform from Agilent Technologies

Nature Methods volume 1, pages 87–89 (2004)Cite this article

The newly introduced Agilent 5100 Automated Lab-on-a-Chip Platform (ALP) offers a high-throughput system to overcome the limitations of slab gel analysis in protein and DNA sizing and quantitation. Agilent, well established in the classical analytical business providing gas and liquid chromatography as well as mass spectrometry instrumentation, has now moved further into the life sciences with its microarray and microfluidic 'Lab-on-a-Chip' solutions.

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Figure 1: Analysis of mPCR using the Automated Lab-on-Chip platform.
Figure 2: (a) Elution profile after separation of a protein mixture by conventional fast-performance liquid chromatography (FPLC).

References

  1. Zink, D., Salowsky, R., Eberhardinger, U., Horeis, B., Gupta, S., Haas, S., Heil, O., Mueller, O., Vingron, M. & Korn, B. Automated microfluidic chip technology to increase the throughput of RT-PCR. Poster presented at Human Proteome Organisation (HUPO) Conference, Berlin, 2004.

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Acknowledgements

The author thank the teams of B. Korn and D. Zink at RZPD Heidelberg, Germany, for taking measurements and for other contributions; A. Shillings and K. Anthony of Gene Expression and Protein Biochemistry at GlaxoSmithKline Research and Development at Harlow, UK; and the Agilent Technology Waldbronn departments in Germany involved in development and support of this work.

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Authors and Affiliations

  1. Martin Greiner is product manager, Automated Lab-on-a-Chip, at Agilent Technology, Hewlett-Packard Str. 8, Waldbronn, D-76337, Germany

    Martin Greiner

  2. Paul Carter is an investigator, Gene Expression & Protein Biochemistry, at Gene Expression and Protein Biochemistry, Discovery Research, GlaxoSmithKline Research and Development, Third Avenue, Harlow, CM19 5AW, Essex, UK

    Paul Carter

  3. Bernhard Kron is chief research officer at RZPD, Im Neuenheimer Feld 580, Heidelberg, D-69120, Germany

    Bernhard Korn

  4. Dorothea Zink is a senior investigator at RZPD, Im Neuenheimer Feld 580, Heidelberg, D-69120, Germany

    Dorothea Zink

Authors
  1. Martin Greiner
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  2. Paul Carter
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  3. Bernhard Korn
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  4. Dorothea Zink
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This article was submitted to Nature Methods by a commercial organization and has not been peer reviewed. Nature Methods takes no responsibility for the accuracy or otherwise of the information provided.

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Greiner, M., Carter, P., Korn, B. et al. New approach to complete automation in sizing and quantitation of DNA and proteins by the Automated Lab-on-a-Chip Platform from Agilent Technologies. Nat Methods 1, 87–89 (2004). https://doi.org/10.1038/nmeth1004-87

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