Visualization of choline-containing phospholipids in cells and in vivo is made possible by the metabolic incorporation of a choline analog with an alkyne handle for click chemistry–based labeling.
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References
Jao, C.Y. et al. Metabolic labeling and direct imaging of choline phospholipids in vivo. Proc. Natl. Acad. Sci. USA 106, 15332–15337 (2009).
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Doerr, A. Illuminating lipids. Nat Methods 6, 696 (2009). https://doi.org/10.1038/nmeth1009-696
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DOI: https://doi.org/10.1038/nmeth1009-696
