Bacterial genomes shuttled into yeast can be easily altered before transplantation back into bacteria.
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References
Lartigue, C. et al. Creating bacterial strains from genomes that have been cloned and engineered in yeast. Science advance online publication (20 August 2009).
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Rusk, N. Engineering bacteria in yeast. Nat Methods 6, 698 (2009). https://doi.org/10.1038/nmeth1009-698
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DOI: https://doi.org/10.1038/nmeth1009-698